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cls cell lines service  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH cls cell lines service
    Cls Cell Lines Service, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+services/U2OS-CRISPR-NUP96-mEGFP+Cells/pmc13186707-293-19-20
    Average 94 stars, based on 28 article reviews
    cls cell lines service - by Bioz Stars, 2026-09
    94/100 stars

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    CRISPR:

    Article Title: Super-Resolution Imaging of Nuclear Pore Responses to Mechanical Stress and Energy Depletion
    Article Snippet: U2OS was obtained from Philip Santangelo (Georgia Institute of Technology, Atlanta, GA, USA), HEK293T/17 cells from ATCC (#CRL11268, Manassas, VA, USA), and TZMbl cells from the BEI Resources (NIAID, NIH; HRP-8129, Manassas, VA, USA). .. U2OS NUP96-SNAP clone #33, in which SNAP was knocked-in at the C-terminus of NUP96 using CRISPR/Cas9 technology, was obtained from Cell Lines Services (CLS GmbH #300444, Eppelheim, Germany; see Thevathasan et al., 2019 [27]). .. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Corning, New York, NY, USA, #10-013- https://doi.org/10.3390/v18020167 CV), supplemented with 10% Fetal Bovine Serum (FBS, Hyclone, #SV30014.03, Logan, UT, USA) and 100 U/mL of Penicillin/Streptomycin (P/S, Gemini Bio-products #400-109, West Sacramento, CA, USA) at 37 ◦C, 5% CO2.

    Article Title: Super-Resolution Imaging of Nuclear Pore Responses to Mechanical Stress and Energy Depletion
    Article Snippet: U2OS was obtained from Philip Santangelo (Georgia Institute of Technology, Atlanta, GA, USA), HEK293T/17 cells from ATCC (# CRL11268 , Manassas, VA, USA), and TZM-bl cells from the BEI Resources (NIAID, NIH; HRP-8129, Manassas, VA, USA). .. U2OS NUP96-SNAP clone #33, in which SNAP was knocked-in at the C-terminus of NUP96 using CRISPR/Cas9 technology, was obtained from Cell Lines Services (CLS GmbH #300444, Eppelheim, Germany; see Thevathasan et al., 2019 [ ]). .. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Corning, New York, NY, USA, #10-013-CV), supplemented with 10% Fetal Bovine Serum (FBS, Hyclone, #SV30014.03, Logan, UT, USA) and 100 U/mL of Penicillin/Streptomycin (P/S, Gemini Bio-products #400-109, West Sacramento, CA, USA) at 37 °C, 5% CO 2 .



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